A cell line authentication test plays an important role in many areas, such as biotech research, drug and vaccine development. It is used to check if the cell samples being studied are correct and pure. Sometimes, cell samples can be wrongly labeled, mixed up, or changed over time. This test helps detect such issues by checking the genetic makeup of the cells. By doing this, specialists can be sure that they are working with the right type of cells. It helps avoid errors in experiments and ensures the results are accurate. This test is particularly helpful when working with animal or human cell lines in labs. It also helps maintain the quality and dependability of the research being done.
Why do Cell Lines Need to be Authenticated?
Cells are the basic building blocks of life. In genetic research and scientific studies, using the correct cell lines is very important. However, these cell lines can sometimes change due to natural or environmental reasons. There are three common issues that can affect cell lines:
- Misidentification
- Genetic Drifting
- Cross-Contamination
- Misidentification – This happens when the wrong cell line is collected from the start. It means the sample was labeled incorrectly or mixed up during the process.
- Genetic Drifting – Over time, cells can change or lose their original structure and become different. This change can affect the results of experiments or research.
- Cross-Contamination – This occurs when cells are accidentally mixed with other cell types, bacteria, or environmental contaminants. This usually happens during storage or lab handling.
These problems can waste a lot of time, money, and effort. To prevent these issues and make sure the cells are genuine, a DNA test is done. It helps confirm the identity of the cell lines for accurate and reliable results.
Method of Authenticating the Cell Line
A cell line DNA test is used to confirm whether the cell lines being used in research are genuine and pure. Below is the step of how this test is done:
- Sample Collecting
The first step is to collect a small portion of the cell sample that needs to be tested. This can be taken directly from the cell culture. The sample should be handled carefully to avoid any contamination from other cells or microbes.
- DNA Extraction
Next, DNA is extracted from the collected cells. This means removing the genetic material, that is, the DNA from the cells using special chemicals and techniques. The DNA is now ready to be analyzed.
- DNA Amplification
Once the DNA is ready, a process called Polymerase Chain Reaction (PCR) is done. PCR is a technique that makes multiple copies of specific DNA regions. In this case, it focuses on Short Tandem Repeats (STRs) — small repeated sequences in DNA that are unique to each cell line.
- DNA Profiling
After PCR, the amplified DNA is analyzed to create a genetic profile of the cell line. This profile acts like a fingerprint — it’s unique for every cell line. It is then compared with the reference profile to check if it matches the correct identity.
- Final Result
The final step is to compare the tested cell line’s DNA profile with the known, correct cell line profiles stored in databases. If the profiles match, the cell line is confirmed as genuine. If not, it could mean the sample is misidentified, contaminated, or has changed over time (genetic drift).
Why Opt for DNA Forensics Laboratory Pvt. Ltd.
When it comes to research and testing, using the correct cell lines is extremely important. Even a small mistake like using the wrong or contaminated cells can lead to wrong results, wasted time, and financial loss. This is why choosing the right lab for the cell line authentication test in India is crucial. DNA Forensics Laboratory Pvt. Ltd. is among the most trusted & reliable names for DNA testing in India. Here’s why you should choose us for this DNA test:
- DNA Forensics Laboratory Pvt. Ltd. uses advanced techniques and globally accepted methods for testing. Our DNA tests are done using STR profiling, which gives accurate and reliable results. We compare the results with global cell line databases to make sure your cell line is exactly what it should be.
- Our lab is NABL-accredited and ISO/IEC 17025 certified, which means we follow strict quality standards. These certifications show that we keep high levels of professionalism and accuracy in every test we perform.
- We have a team of skilled scientists, geneticists, & technicians who are well-trained in handling sensitive samples. Our experience ensures that the test is done correctly and the results are reliable.
- Our laboratory is equipped with cutting-edge technology for DNA extraction, amplification, and profiling. This helps in faster and more precise testing. The equipment is regularly updated and maintained to meet international testing standards.
- Time is crucial, especially in research work. We offer quick testing services without compromising on quality. You can expect timely reports so your research or project can move forward without delays.
- We have 400+ collection centres nationally and internationally. No matter where you are in India, the lab offers sample collection services across the country. You can send your samples easily without worrying about location barriers.
- We follow strict guidelines to maintain the privacy of your data and results. Your samples and reports are handled with full confidentiality and are shared only with you, the authorized persons. We also use secure systems and encryption methods to ensure your sensitive details remain protected at every stage of the testing process.
- Here, you get accurate results, expert support, and quick service – all at a competitive price.
- We provide tailored services to suit the specific needs of your research or organization. Whether you’re testing one sample or managing a large research project, our team provides flexible testing options based on your goals.
To learn more or to book a cell line test in India, talk to our customer service representatives at +91 8010177771. You can also chat with us on WhatsApp at + 91 9266615552.
Frequently Asked Questions About Cell Line:
Q1. What is cell line authentication?
Verifies the identity and purity of a cultured cell line using DNA profiling—ensuring you work with the correct, uncontaminated cells.
Q2. Why is cell line authentication important?
It helps identify errors, like misidentification, genetic drift, and cross-contamination of cell lines under study—safeguarding research accuracy and reproducibility, essential in drug development and academia.
Q3. How is a cell line authentication test done in India?
DNA Forensics Laboratory analyzes STR (Short Tandem Repeat) markers from your sample and compares them to reference databases to confirm identity.
Q4. When should cell line authentication be done?
Before starting experiments, after thawing, after extended culture, or when unexpected results show up—periodic checks are best.
Q5. Is cell line authentication mandatory in India?
It’s not legally mandatory yet, but highly recommended by scientific journals and research institutions for reliable data integrity.
Q6. What problems can occur if cell lines aren’t authenticated?
Misidentified or cross-contaminated lines lead to invalid results, wasted time, failed experiments, and irreproducible findings.
Q7. How much does cell line authentication cost in India?
The price of the test is 14160 INR, which may be subject to change depending on the cell type, number of cell lines, depth of analysis, and other factors.
Q8. How long does it take to get results?
You can expect a full report within 10-15 working days once samples reach the lab.
Q9. What samples are needed?
A small pellet or pellet-freeze of your cultured cells (minimum 1 million cells) is usually sufficient for STR profiling.
Q10. Which labs offer this testing in India?
DNA Forensics Laboratory Pvt. Ltd. from its head office in Delhi and through 400+ collection centers across India, provides validated cell line authentication test with global standards.
Q11. Is cell line authentication confidential?
Yes—your data remains fully confidential, and only you receive the detailed report and certification.
Q12. Can this test detect contamination?
Absolutely—STR profiling can identify cross-contamination, helping you ensure cell line purity and trust in results.
Q13. Are the tests internationally accepted?
Yes, our STR-based analysis aligns with international standards and is accepted by journals and collaborations worldwide.
Q14. Can I get a certificate for authentication?
Yes—DNA Forensics Laboratory issues a formal authentication certificate upon successful validation of your cell line.
